UniProt functional annotation for P05050



	UniProt functional annotation for P05050

UniProt code: P05050.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: Dioxygenase that repairs alkylated DNA and RNA containing 3- methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha- ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents). {ECO:0000269|PubMed:12226668, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:16482161, ECO:0000269|PubMed:19706517, ECO:0000269|PubMed:20084272, ECO:0000269|PubMed:21068844}.

Catalytic activity: Reaction=2-oxoglutarate + a methylated nucleobase within DNA + O2 = a nucleobase within DNA + CO2 + formaldehyde + succinate; Xref=Rhea:RHEA:30299, Rhea:RHEA-COMP:12192, Rhea:RHEA-COMP:12193, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:16842, ChEBI:CHEBI:30031, ChEBI:CHEBI:32875, ChEBI:CHEBI:64428; EC=1.14.11.33; Evidence={ECO:0000269|PubMed:12226668, ECO:0000269|PubMed:20084272, ECO:0000269|PubMed:21068844};

Cofactor: Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000255|PROSITE-ProRule:PRU00805, ECO:0000269|PubMed:16482161, ECO:0000269|PubMed:19706517, ECO:0000269|PubMed:20084272, ECO:0000269|PubMed:21068844}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE- ProRule:PRU00805, ECO:0000269|PubMed:16482161, ECO:0000269|PubMed:19706517, ECO:0000269|PubMed:20084272, ECO:0000269|PubMed:21068844};

Similarity: Belongs to the alkB family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		Dioxygenase that repairs alkylated DNA and RNA containing 3- methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha- ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents). {ECO:0000269\|PubMed:12226668, ECO:0000269\|PubMed:12594517, ECO:0000269\|PubMed:16482161, ECO:0000269\|PubMed:19706517, ECO:0000269\|PubMed:20084272, ECO:0000269\|PubMed:21068844}.


Catalytic activity:		Reaction=2-oxoglutarate + a methylated nucleobase within DNA + O2 = a nucleobase within DNA + CO2 + formaldehyde + succinate; Xref=Rhea:RHEA:30299, Rhea:RHEA-COMP:12192, Rhea:RHEA-COMP:12193, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:16842, ChEBI:CHEBI:30031, ChEBI:CHEBI:32875, ChEBI:CHEBI:64428; EC=1.14.11.33; Evidence={ECO:0000269\|PubMed:12226668, ECO:0000269\|PubMed:20084272, ECO:0000269\|PubMed:21068844};
Cofactor:		Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000255\|PROSITE-ProRule:PRU00805, ECO:0000269\|PubMed:16482161, ECO:0000269\|PubMed:19706517, ECO:0000269\|PubMed:20084272, ECO:0000269\|PubMed:21068844}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255\|PROSITE- ProRule:PRU00805, ECO:0000269\|PubMed:16482161, ECO:0000269\|PubMed:19706517, ECO:0000269\|PubMed:20084272, ECO:0000269\|PubMed:21068844};
Similarity:		Belongs to the alkB family. {ECO:0000305}.