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UniProt functional annotation for Q9I6K2 | ||
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| UniProt code: Q9I6K2. |
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| Organism: | |
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. |
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| Function: | |
Catalyzes the hydrolysis of 3-guanidinopropanoate to beta- alanine and urea. Possesses low activity against 4-guanidinobutanoate. Has no activity against arginine and agmatine. {ECO:0000269|PubMed:21600989}. |
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| Catalytic activity: | |
Reaction=3-guanidinopropanoate + H2O = beta-alanine + urea; Xref=Rhea:RHEA:16029, ChEBI:CHEBI:15377, ChEBI:CHEBI:16199, ChEBI:CHEBI:57593, ChEBI:CHEBI:57966; EC=3.5.3.17; Evidence={ECO:0000269|PubMed:21600989}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|PROSITE-ProRule:PRU00742, ECO:0000269|PubMed:21600989}; Note=Binds 2 manganese ions per subunit. {ECO:0000255|PROSITE- ProRule:PRU00742, ECO:0000269|PubMed:21600989}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=12.8 mM for 3-guanidinopropanoate {ECO:0000269|PubMed:21600989}; KM=37.3 mM for 4-guanidinobutanoate {ECO:0000269|PubMed:21600989}; Note=kcat is 106 s(-1) with 3-guanidinopropanoate as substrate. kcat is 57.6 s(-1) for 4-guanidinobutanoate as substrate.; |
| Subunit: | |
Homohexamer. {ECO:0000269|PubMed:21600989}. |
| Similarity: | |
Belongs to the arginase family. Agmatinase subfamily. {ECO:0000255|PROSITE-ProRule:PRU00742}. |
Annotations taken from UniProtKB at the EBI.