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PDBsum entry 3mzs
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Oxidoreductase
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PDB id
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3mzs
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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Crystal structure of cytochrome p450 cyp11a1 in complex with 22- hydroxy-cholesterol
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Structure:
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Cholesterol side-chain cleavage enzyme. Chain: a, b, c, d. Synonym: cytochrome p450 11a1, cypxia1, cytochrome p450(scc), cholesterol desmolase. Engineered: yes
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Source:
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Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913. Gene: cyp11a1. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.50Å
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R-factor:
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0.266
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R-free:
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0.281
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Authors:
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C.D.Stout,A.Annalora,N.Mast,I.Pikuleva
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Key ref:
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N.Mast
et al.
(2011).
Structural basis for three-step sequential catalysis by the cholesterol side chain cleavage enzyme CYP11A1.
J Biol Chem,
286,
5607-5613.
PubMed id:
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Date:
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12-May-10
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Release date:
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15-Dec-10
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PROCHECK
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Headers
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References
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P00189
(CP11A_BOVIN) -
Cholesterol side-chain cleavage enzyme, mitochondrial from Bos taurus
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Seq:
Struc:
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520 a.a.
470 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.1.14.15.6
- cholesterol monooxygenase (side-chain-cleaving).
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Reaction:
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6 reduced [adrenodoxin] + cholesterol + 3 O2 + 6 H+ = 4-methylpentanal + pregnenolone + 6 oxidized [adrenodoxin] + 4 H2O
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6
×
reduced [adrenodoxin]
Bound ligand (Het Group name = )
matches with 96.55% similarity
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cholesterol
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3
×
O2
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+
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6
×
H(+)
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=
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4-methylpentanal
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pregnenolone
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+
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6
×
oxidized [adrenodoxin]
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+
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4
×
H2O
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Cofactor:
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Heme-thiolate
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Biol Chem
286:5607-5613
(2011)
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PubMed id:
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Structural basis for three-step sequential catalysis by the cholesterol side chain cleavage enzyme CYP11A1.
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N.Mast,
A.J.Annalora,
D.T.Lodowski,
K.Palczewski,
C.D.Stout,
I.A.Pikuleva.
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ABSTRACT
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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N.M.DeVore,
and
E.E.Scott
(2012).
Structures of cytochrome P450 17A1 with prostate cancer drugs abiraterone and TOK-001.
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Nature,
482,
116-119.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
