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UniProt functional annotation for P26935 | ||
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| UniProt code: P26935. |
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| Organism: | |
Bacillus subtilis (strain 168). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. |
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| Function: | |
Involved in the oxidation of myo-inositol (MI) and D-chiro- inositol (DCI) to 2-keto-myo-inositol (2KMI or 2-inosose) and 1-keto-D- chiro-inositol (1KDCI), respectively. Can also use D-glucose and D- xylose, and shows a trace of activity with D-ribose and D-fructose. {ECO:0000269|PubMed:112095, ECO:0000269|PubMed:16461681}. |
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| Catalytic activity: | |
Reaction=myo-inositol + NAD(+) = H(+) + NADH + scyllo-inosose; Xref=Rhea:RHEA:16949, ChEBI:CHEBI:15378, ChEBI:CHEBI:17268, ChEBI:CHEBI:17811, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.18; Evidence={ECO:0000269|PubMed:16461681}; |
| Catalytic activity: | |
Reaction=1D-chiro-inositol + NAD(+) = H(+) + NADH + scyllo-inosine; Xref=Rhea:RHEA:25832, ChEBI:CHEBI:15378, ChEBI:CHEBI:27372, ChEBI:CHEBI:50920, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.369; Evidence={ECO:0000269|PubMed:16461681}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.036 mM for NADH (in the presence of 5 mM 2-inosose at 25 degrees Celsius and pH 7) {ECO:0000269|PubMed:112095}; KM=0.23 mM for NAD (in the presence of 40 mM myo-inositol at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; KM=1.61 mM for 2-inosose (in the presence of 0.1 mM NADH at 25 degrees Celsius and pH 7) {ECO:0000269|PubMed:112095}; KM=18.2 mM for myo-inositol (in the presence of 0.5 mM NAD at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; KM=55.6 mM for alpha-D-glucose (in the presence of 0.5 mM NAD at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; KM=167 mM for D-glucose (in the presence of 0.5 mM NAD at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; KM=190 mM for D-xylose (in the presence of 0.5 mM NAD at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; Vmax=42 umol/min/mg enzyme for 2-inosose reduction reaction (at 25 degrees Celsius and pH 7) {ECO:0000269|PubMed:112095}; Vmax=21 umol/min/mg enzyme for myo-inositol oxidation reaction (at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; Vmax=13.5 umol/min/mg enzyme with for alpha-D-glucose oxidation reaction (at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; Vmax=7.3 umol/min/mg enzyme for D-glucose oxidation reaction (at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; Vmax=6.7 umol/min/mg enzyme for D-xylose oxidation reaction (at 25 degrees Celsius and pH 9) {ECO:0000269|PubMed:112095}; pH dependence: Optimum pH is 9.5 for inositol 2-dehydrogenase activity. {ECO:0000269|PubMed:112095}; |
| Pathway: | |
Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 1/7. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:112095}. |
| Induction: | |
By inositol. Subjected to catabolite repression. {ECO:0000269|PubMed:1761221}. |
| Similarity: | |
Belongs to the Gfo/Idh/MocA family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.