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PDBsum entry 3m2d
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Oxidoreductase
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PDB id
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3m2d
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References listed in PDB file
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Key reference
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Title
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Crystallographic and single-Crystal spectral analysis of the peroxidase ferryl intermediate.
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Authors
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Y.T.Meharenna,
T.Doukov,
H.Li,
S.M.Soltis,
T.L.Poulos.
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Ref.
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Biochemistry, 2010,
49,
2984-2986.
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PubMed id
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Abstract
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The ferryl [Fe(IV)O] intermediate is important in many heme enzymes, and thus,
the precise nature of the Fe(IV)-O bond is critical in understanding enzymatic
mechanisms. The 1.40 A crystal structure of cytochrome c peroxidase Compound I
has been determined as a function of X-ray dose while the visible spectrum was
being monitored. The Fe-O bond increases in length from 1.73 A in the low-X-ray
dose structure to 1.90 A in the high-dose structure. The low-dose structure
correlates well with an Fe(IV) horizontal lineO bond, while we postulate that
the high-dose structure is the cryo-trapped Fe(III)-OH species previously
thought to be an Fe(IV)-OH species.
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