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UniProt functional annotation for P36136 | ||
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| UniProt code: P36136. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Sedoheptulose 1,7-bisphosphatase involved in riboneogenesis. Dephosphorylates sedoheptulose 1,7-bisphosphate (SBP), which is converted via the non-oxidative pentose phosphate pathway to ribose-5- phosphate. Has a fructose 1,6-bisphosphatase activity in vitro, but this is probably not biologically relevant, since deletion does not affect fructose 1,6-biphosphate (FBP) levels. {ECO:0000269|PubMed:21663798}. |
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| Catalytic activity: | |
Reaction=H2O + sedoheptulose 1,7-bisphosphate = D-sedoheptulose 7- phosphate + phosphate; Xref=Rhea:RHEA:17461, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:57483, ChEBI:CHEBI:58335; EC=3.1.3.37; Evidence={ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=34 uM for sedoheptulose 1,7-bisphosphate (SBP) {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; KM=0.2 mM for fructose 1,6-bisphosphate (FBP) {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; KM=0.7 mM for fructose 1,6-bisphosphate (in the presence of 1 mM fructose 2,6-bisphosphate) {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; KM=2.3 mM for fructose 1,6-bisphosphate (in the presence of 2 mM fructose 2,6-bisphosphate) {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; KM=1.4 mM for fructose 1-phosphate {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; KM=1.6 mM for glyceraldehyde 3-phosphate {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; KM=0.3 mM for erythrose 4-phosphate {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; Vmax=7.8 umol/min/mg enzyme toward sedoheptulose 1,7-bisphosphate (SBP) {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; Vmax=8.7 umol/min/mg enzyme toward fructose 1,6-bisphosphate (FBP) {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; Vmax=7.3 umol/min/mg enzyme toward fructose 1,6-bisphosphate (in the presence of 1 mM fructose 2,6-bisphosphate) {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; Vmax=8.9 umol/min/mg enzyme toward fructose 1,6-bisphosphate,(in the presence of 1 mM fructose 2,6-bisphosphate) {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; Vmax=7.3 umol/min/mg enzyme toward fructose 1-phosphate {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; Vmax=1 umol/min/mg enzyme toward glyceraldehyde 3-phosphate {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; Vmax=0.6 umol/min/mg enzyme toward erythrose 4-phosphate {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; pH dependence: Optimum pH is 6.5. {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:20427268, ECO:0000269|PubMed:21663798}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}. |
| Induction: | |
Expression is correlated with levels of ribosomal proteins, thus periodic SHB17 expression coincides with the peak demand for ribose phosphate that occurs during ribosome synthesis. {ECO:0000269|PubMed:21663798}. |
| Miscellaneous: | |
Present with 11500 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the phosphoglycerate mutase family. SHB17 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.