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UniProt functional annotation for Q8XIQ9 | ||
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| UniProt code: Q8XIQ9. |
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| Organism: | |
Clostridium perfringens (strain 13 / Type A). |
| Taxonomy: | |
Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium. |
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| Catalytic activity: | |
Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=3.6.1.1; Evidence={ECO:0000269|PubMed:20303981, ECO:0000269|PubMed:22348476}; |
| Cofactor: | |
Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:22348476}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:22348476}; Note=Binds tightly a transition metal ion; prefers Co(2+) over Mn(2+). {ECO:0000269|PubMed:22348476}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:22348476}; Note=Mg(2+) ions are required for optimal catalytic activity. {ECO:0000269|PubMed:22348476}; |
| Activity regulation: | |
Inhibited by AMP and ADP with 25% and 35% of activity remaining, respectively, at saturating conditions. Activated 5-fold by diadenosine polyphosphates(Ap[n]A) with n>2 (Ap3A, Ap4A, Ap5A, Ap6A) at saturating conditions. {ECO:0000269|PubMed:20303981, ECO:0000269|PubMed:22348476}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=50 uM for pyrophosphate {ECO:0000269|PubMed:22348476}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:20303981}. |
| Similarity: | |
Belongs to the PPase family. {ECO:0000255}. |
Annotations taken from UniProtKB at the EBI.