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UniProt functional annotation for Q1EMV2 | ||
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| UniProt code: Q1EMV2. |
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| Organism: | |
Streptomyces cattleya. |
| Taxonomy: | |
Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; Streptomyces. |
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| Function: | |
Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate. {ECO:0000269|PubMed:16720268, ECO:0000269|PubMed:20836570}. |
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| Catalytic activity: | |
Reaction=fluoroacetyl-CoA + H2O = CoA + fluoroacetate + H(+); Xref=Rhea:RHEA:28542, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18172, ChEBI:CHEBI:57287, ChEBI:CHEBI:61623; EC=3.1.2.29; Evidence={ECO:0000269|PubMed:16720268, ECO:0000269|PubMed:20836570}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=30 uM for fluoroacetyl-CoA (at pH 8 and 25 degrees Celsius) {ECO:0000269|PubMed:16720268, ECO:0000269|PubMed:20430898}; KM=8 uM for fluoroacetyl-CoA (at pH 7.6 and 25 degrees Celsius) {ECO:0000269|PubMed:20836570}; KM=2.1 mM for acetyl-CoA (at pH 7.6 and 25 degrees Celsius) {ECO:0000269|PubMed:20836570}; Vmax=15 umol/min/mg enzyme with fluoroacetyl-CoA as substrate (at pH 8 and 25 degrees Celsius) {ECO:0000269|PubMed:16720268}; Note=Kcat is 390 sec(-1) with fluoroacetyl-CoA as substrate and 0.06 sec(-1) with acetyl-CoA (at pH 7.6 and 25 degrees Celsius, PubMed:20836570). Kcat is 0.044 sec(-1) with fluoroacetyl-CoA as substrate (PubMed:20430898). {ECO:0000269|PubMed:20430898}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:20430898, ECO:0000269|PubMed:20836570}. |
Annotations taken from UniProtKB at the EBI.