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PDBsum entry 3knh
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Ribosome/antibiotic
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PDB id
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3knh
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Contents |
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235 a.a.
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207 a.a.
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208 a.a.
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151 a.a.
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101 a.a.
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155 a.a.
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138 a.a.
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127 a.a.
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99 a.a.
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119 a.a.
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125 a.a.
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120 a.a.
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60 a.a.
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88 a.a.
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84 a.a.
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100 a.a.
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70 a.a.
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79 a.a.
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99 a.a.
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25 a.a.
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References listed in PDB file
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Key reference
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Title
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The structures of the anti-Tuberculosis antibiotics viomycin and capreomycin bound to the 70s ribosome.
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Authors
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R.E.Stanley,
G.Blaha,
R.L.Grodzicki,
M.D.Strickler,
T.A.Steitz.
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Ref.
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Nat Struct Biol, 2010,
17,
289-293.
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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Viomycin and capreomycin belong to the tuberactinomycin family of antibiotics,
which are among the most effective antibiotics against multidrug-resistant
tuberculosis. Here we present two crystal structures of the 70S ribosome in
complex with three tRNAs and bound to either viomycin or capreomycin at 3.3- and
3.5-A resolution, respectively. Both antibiotics bind to the same site on the
ribosome, which lies at the interface between helix 44 of the small ribosomal
subunit and helix 69 of the large ribosomal subunit. The structures of these
complexes suggest that the tuberactinomycins inhibit translocation by
stabilizing the tRNA in the A site in the pretranslocation state. In addition,
these structures show that the tuberactinomycins bind adjacent to the binding
sites for the paromomycin and hygromycin B antibiotics, which may enable the
development of new derivatives of tuberactinomycins that are effective against
drug-resistant strains.
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