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PDBsum entry 3ju4
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References listed in PDB file
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Key reference
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Title
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Structure analysis of endosialidase nf at 0.98 a resolution.
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Authors
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E.C.Schulz,
P.Neumann,
R.Gerardy-Schahn,
G.M.Sheldrick,
R.Ficner.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2010,
66,
176-180.
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PubMed id
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Abstract
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Endosialidase NF (endoNF) is a bacteriophage-derived endosialidase that
specifically degrades alpha-2,8-linked polysialic acid. The structure of a new
crystal form of endoNF in complex with sialic acid has been refined at 0.98 A
resolution. The 210 kDa homotrimeric multi-domain enzyme displays outstanding
stability and resistance to SDS. Even at atomic resolution, only a minor
fraction of side chains possess alternative conformations. However, multiple
conformations of an active-site residue imply that it has an important catalytic
function in the cleavage mechanism of polysialic acid.
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