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PDBsum entry 3ipr
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References listed in PDB file
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Key reference
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Title
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Structure of the enterococcus faecalis eiia(gnt) pts component.
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Authors
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S.Reinelt,
B.Koch,
M.Hothorn,
W.Hengstenberg,
S.Welti,
K.Scheffzek.
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Ref.
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Biochem Biophys Res Commun, 2009,
388,
626-629.
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PubMed id
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Abstract
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In Eubacteria, the utilization of a number of extracellular carbohydrates is
mediated by sugar specific phosphoenolepyruvate (PEP) dependent sugar
phosphotransferase systems (PTSs), which simultaneously import und phosphorylate
their target sugars. Here, we report the crystal structure of the EIIA(gnt)
component of the so far little investigated Enterococcus faecalis gluconate
specific PTS. The crystal structure shows a tightly interacting dimer of
EIIA(gnt) which is structurally similar to the related EIIA(man) from
Escherichia coli. Homology modeling of E. faecalis HPr, EIIB(man) and their
complexes with EIIA(man) suggests that despite moderate sequence identity
between EIIA(man) and EIIA(gnt), the active sites closely match the situation
observed in the E. coli system with His-9 of EIIA(gnt) being the likely
phosphoryl group carrier. We therefore propose that the phosphoryl transfer
reactions involving EIIA(gnt) proceed according to a mechanism analog to the one
described for E. coli EIIA(man).
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