PDBsum entry 3i4t

Structural genomics, unknown function

PDB id: 3i4t

Contents

Protein chain

252 a.a. *

Waters ×65

* Residue conservation analysis

PDB id:

3i4t

Name:

Structural genomics, unknown function

Title:

Crystal structure of putative diphthine synthase from entamoeba histolytica

Structure:

Diphthine synthase. Chain: a. Engineered: yes. Mutation: yes

Source:

Entamoeba histolytica. Organism_taxid: 5759. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.49Å R-factor: 0.226 R-free: 0.273

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

T.E.Edwards et al. Crystal structure of putative diphthine synthase from entamoeba histolytica. To be published, .

Date:

02-Jul-09 Release date: 28-Jul-09

Protein chain

C4LWZ7  (C4LWZ7_ENTHI) -  diphthine methyl ester synthase from Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

Seq: 271 a.a.

Struc: 252 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.1.1.314 - diphthine methyl ester synthase.
• Reaction: 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site