UniProt functional annotation for Q7SIG4



	UniProt functional annotation for Q7SIG4

UniProt code: Q7SIG4.

Organism: Loligo vulgaris (Common European squid).

Taxonomy: Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Cephalopoda; Coleoidea; Decapodiformes; Teuthida; Myopsina; Loliginidae; Loligo.

Function: Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus- halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases). {ECO:0000269|PubMed:15966726}.

Catalytic activity: Reaction=diisopropyl fluorophosphate + H2O = diisopropyl phosphate + fluoride + 2 H(+); Xref=Rhea:RHEA:24100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17051, ChEBI:CHEBI:17941, ChEBI:CHEBI:57896; EC=3.1.8.2; Evidence={ECO:0000269|PubMed:11171055, ECO:0000269|PubMed:11295437};

Cofactor: Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:11171055, ECO:0000269|PubMed:11435114, ECO:0000269|PubMed:14501113}; Note=Binds 2 calcium ions per subunit. {ECO:0000269|PubMed:11171055, ECO:0000269|PubMed:11435114, ECO:0000269|PubMed:14501113};

Activity regulation: Inhibited by chelating agents. {ECO:0000269|PubMed:11171055}.

Biophysicochemical properties: Kinetic parameters: KM=0.54 mM for diisopropyl-fluorophosphate (in the presence of 10 mM NaCl) {ECO:0000269|PubMed:11295437}; KM=0.42 mM for diisopropyl-fluorophosphate (in the presence of 100 mM NaCl) {ECO:0000269|PubMed:11295437}; KM=0.34 mM for diisopropyl-fluorophosphate (in the presence of 200 mM NaCl) {ECO:0000269|PubMed:11295437}; KM=0.25 mM for diisopropyl-fluorophosphate (in the presence of 500 mM NaCl) {ECO:0000269|PubMed:11295437}; KM=0.17 mM for diisopropyl-fluorophosphate (in the presence of 1000 mM NaCl) {ECO:0000269|PubMed:11295437}; Vmax=130 umol/min/mg enzyme (in the presence of 10 mM NaCl) {ECO:0000269|PubMed:11295437}; Vmax=170 umol/min/mg enzyme (in the presence of 100 mM NaCl) {ECO:0000269|PubMed:11295437}; Vmax=307 umol/min/mg enzyme (in the presence of 200 mM NaCl) {ECO:0000269|PubMed:11295437}; Vmax=326 umol/min/mg enzyme (in the presence of 500 mM NaCl) {ECO:0000269|PubMed:11295437}; Vmax=214 umol/min/mg enzyme (in the presence of 1000 mM NaCl) {ECO:0000269|PubMed:11295437}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:11295437}; Temperature dependence: Optimum temperature is 35 degrees Celsius. {ECO:0000269|PubMed:11295437};

Subunit: Monomer. {ECO:0000269|PubMed:11435114, ECO:0000269|PubMed:14501113}.

Biotechnology: Has potential application in bio-remediation by detoxification of organo-phosphates used in insecticides or nerve agents used in chemical warfare such as soman, tabun and sarin. {ECO:0000269|PubMed:11134940}.

Annotations taken from UniProtKB at the EBI.


Organism:		Loligo vulgaris (Common European squid).
Taxonomy:		Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Cephalopoda; Coleoidea; Decapodiformes; Teuthida; Myopsina; Loliginidae; Loligo.



Function:		Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus- halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases). {ECO:0000269\|PubMed:15966726}.


Catalytic activity:		Reaction=diisopropyl fluorophosphate + H2O = diisopropyl phosphate + fluoride + 2 H(+); Xref=Rhea:RHEA:24100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17051, ChEBI:CHEBI:17941, ChEBI:CHEBI:57896; EC=3.1.8.2; Evidence={ECO:0000269\|PubMed:11171055, ECO:0000269\|PubMed:11295437};
Cofactor:		Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269\|PubMed:11171055, ECO:0000269\|PubMed:11435114, ECO:0000269\|PubMed:14501113}; Note=Binds 2 calcium ions per subunit. {ECO:0000269\|PubMed:11171055, ECO:0000269\|PubMed:11435114, ECO:0000269\|PubMed:14501113};
Activity regulation:		Inhibited by chelating agents. {ECO:0000269\|PubMed:11171055}.
Biophysicochemical properties:		Kinetic parameters: KM=0.54 mM for diisopropyl-fluorophosphate (in the presence of 10 mM NaCl) {ECO:0000269\|PubMed:11295437}; KM=0.42 mM for diisopropyl-fluorophosphate (in the presence of 100 mM NaCl) {ECO:0000269\|PubMed:11295437}; KM=0.34 mM for diisopropyl-fluorophosphate (in the presence of 200 mM NaCl) {ECO:0000269\|PubMed:11295437}; KM=0.25 mM for diisopropyl-fluorophosphate (in the presence of 500 mM NaCl) {ECO:0000269\|PubMed:11295437}; KM=0.17 mM for diisopropyl-fluorophosphate (in the presence of 1000 mM NaCl) {ECO:0000269\|PubMed:11295437}; Vmax=130 umol/min/mg enzyme (in the presence of 10 mM NaCl) {ECO:0000269\|PubMed:11295437}; Vmax=170 umol/min/mg enzyme (in the presence of 100 mM NaCl) {ECO:0000269\|PubMed:11295437}; Vmax=307 umol/min/mg enzyme (in the presence of 200 mM NaCl) {ECO:0000269\|PubMed:11295437}; Vmax=326 umol/min/mg enzyme (in the presence of 500 mM NaCl) {ECO:0000269\|PubMed:11295437}; Vmax=214 umol/min/mg enzyme (in the presence of 1000 mM NaCl) {ECO:0000269\|PubMed:11295437}; pH dependence: Optimum pH is 8.0. {ECO:0000269\|PubMed:11295437}; Temperature dependence: Optimum temperature is 35 degrees Celsius. {ECO:0000269\|PubMed:11295437};
Subunit:		Monomer. {ECO:0000269\|PubMed:11435114, ECO:0000269\|PubMed:14501113}.
Biotechnology:		Has potential application in bio-remediation by detoxification of organo-phosphates used in insecticides or nerve agents used in chemical warfare such as soman, tabun and sarin. {ECO:0000269\|PubMed:11134940}.