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UniProt functional annotation for O44249 | ||
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| UniProt code: O44249. |
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| Organism: | |
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm). |
| Taxonomy: | |
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea; Sphingidae; Sphinginae; Sphingini; Manduca. |
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| Function: | |
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone. Binds to the surface of hemocytes and is involved in hemocyte melanization. {ECO:0000269|PubMed:16291091, ECO:0000305}. |
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| Catalytic activity: | |
Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57924; EC=1.14.18.1; |
| Catalytic activity: | |
Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924, ChEBI:CHEBI:58315; EC=1.14.18.1; |
| Cofactor: | |
Name=Cu(2+); Xref=ChEBI:CHEBI:29036; Evidence={ECO:0000269|PubMed:19805072}; Note=Binds 2 copper ions per subunit. {ECO:0000269|PubMed:19805072}; |
| Activity regulation: | |
Activated by immulectin and lipopolysaccharide. {ECO:0000269|PubMed:10436935}. |
| Subunit: | |
Heterodimer. Forms a complex with an interleukin 1-like protein as a consequence of a host defense response. {ECO:0000269|PubMed:19805072, ECO:0000269|PubMed:8626641, ECO:0000269|PubMed:9474780}. |
| Subcellular location: | |
Secreted {ECO:0000269|PubMed:9474780}. |
| Tissue specificity: | |
Synthesized by oenocytoids, a type of hemocyte, and released into the hemolymph plasma. {ECO:0000269|PubMed:9474780}. |
| Ptm: | |
The N-terminus is blocked. {ECO:0000269|PubMed:9474780}. |
| Mass spectrometry: | |
Mass=78904; Method=MALDI; Evidence={ECO:0000269|PubMed:9474780}; |
| Similarity: | |
Belongs to the tyrosinase family. {ECO:0000255}. |
Annotations taken from UniProtKB at the EBI.