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UniProt functional annotation for P00720 | ||
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| UniProt code: P00720. |
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| Organism: | |
Enterobacteria phage T4 (Bacteriophage T4). |
| Taxonomy: | |
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus. |
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| Function: | |
Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer. {ECO:0000255|HAMAP-Rule:MF_04110, ECO:0000269|PubMed:22389108}. |
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| Catalytic activity: | |
Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17; Evidence={ECO:0000255|HAMAP-Rule:MF_04110, ECO:0000269|PubMed:4865643}; |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 7.3. {ECO:0000269|PubMed:4865643}; |
| Subcellular location: | |
Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04110}. Note=The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane. {ECO:0000255|HAMAP-Rule:MF_04110}. |
| Induction: | |
Expressed late in the viral replication. |
| Similarity: | |
Belongs to the glycosyl hydrolase 24 family. {ECO:0000255|HAMAP-Rule:MF_04110}. |
Annotations taken from UniProtKB at the EBI.