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PDBsum entry 3grf
Go to PDB code: 
protein metals links
Transferase PDB id
3grf

 

 

 

 

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Contents
Protein chain
304 a.a. *
Metals
_NI
Waters ×267
* Residue conservation analysis
PDB id:
3grf
Name: Transferase
Title: X-ray structure of ornithine transcarbamoylase from giardia lamblia
Structure: Ornithine carbamoyltransferase. Chain: a. Engineered: yes
Source: Giardia intestinalis. Organism_taxid: 5741. Strain: wb. Gene: otc. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.00Å     R-factor:   0.197     R-free:   0.255
Authors: A.Galkin,O.Herzberg
Key ref:
A.Galkin et al. (2009). X-ray structure and kinetic properties of ornithine transcarbamoylase from the human parasite Giardia lamblia. Proteins, 76, 1049-1053. PubMed id: 19507245 DOI: 10.1002/prot.22469
Date:
25-Mar-09     Release date:   30-Jun-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
O76458  (O76458_GIAIN) -  ornithine carbamoyltransferase from Giardia intestinalis
Seq:
Struc: 		327 a.a.
304 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.2.1.3.3  - ornithine carbamoyltransferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway: 		Urea Cycle and Arginine Biosynthesis
      Reaction: carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H+
carbamoyl phosphate
 + L-ornithine
 = L-citrulline
 + phosphate
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
DOI no: 10.1002/prot.22469 Proteins 76:1049-1053 (2009)
PubMed id: 19507245  
 
 
X-ray structure and kinetic properties of ornithine transcarbamoylase from the human parasite Giardia lamblia.
A.Galkin, L.Kulakova, R.Wu, M.Gong, D.Dunaway-Mariano, O.Herzberg.
 
  ABSTRACT  
 
No abstract given.

 
  Selected figure(s)  
 
Figure 1.
Figure 1. Overall fold, active site architecture of glOTC, and sequence alignment of glOTC and hOTC. (A) Ribbon diagram representation of the protein trimer. The Ni^2+ ion at the center of the trimer is shown as magenta sphere. (B) Stereoscopic view of the glOTC active site superposed with the hOTC/PALO active site. The carbon atoms are colored green (glOTC) and magenta (hOTC). Other atomic colors are as follows: oxygen, red; nitrogen, blue; phosphor, orange; and sulfur, yellow. Black residue labels correspond to glOTC, except that the two residues labeled in magenta color (His117 of a neighboring subunit and Met268) are hOTC residues that are disordered in the glOTC structure (Ser82 and Tyr245, respectively). (C) Sequence alignment of glOTC and hOTC. Identical residues are blocked in blue and residues surrounding the active site are indicated by red triangles.
 
  The above figure is reprinted from an Open Access publication published by John Wiley & Sons, Inc.: Proteins (2009, 76, 1049-1053) copyright 2009.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
  20383005 A.Galkin, L.Kulakova, R.Wu, T.E.Nash, D.Dunaway-Mariano, and O.Herzberg (2010).
X-ray structure and characterization of carbamate kinase from the human parasite Giardia lamblia.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 66, 386-390.
PDB code: 3kzf
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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