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PDBsum entry 3glc
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References listed in PDB file
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Key reference
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Title
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The crystal structure of the escherichia coli autoinducer-2 processing protein lsrf.
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Authors
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Z.Diaz,
K.B.Xavier,
S.T.Miller.
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Ref.
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Plos One, 2009,
4,
e6820.
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PubMed id
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Abstract
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Many bacteria produce and respond to the quorum sensing signal autoinducer-2
(AI-2). Escherichia coli and Salmonella typhimurium are among the species with
the lsr operon, an operon containing AI-2 transport and processing genes that
are up regulated in response to AI-2. One of the Lsr proteins, LsrF, has been
implicated in processing the phosphorylated form of AI-2. Here, we present the
structure of LsrF, unliganded and in complex with two phospho-AI-2 analogues,
ribose-5-phosphate and ribulose-5-phosphate. The crystal structure shows that
LsrF is a decamer of (alphabeta)(8)-barrels that exhibit a previously unseen
N-terminal domain swap and have high structural homology with aldolases that
process phosphorylated sugars. Ligand binding sites and key catalytic residues
are structurally conserved, strongly implicating LsrF as a class I aldolase.
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