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UniProt functional annotation for P0AEZ1 | ||
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| UniProt code: P0AEZ1. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Methylenetetrahydrofolate reductase required to generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine. {ECO:0000269|PubMed:14275142}. |
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| Catalytic activity: | |
Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH; Xref=Rhea:RHEA:19817, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636, ChEBI:CHEBI:18608, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.5.1.20; Evidence={ECO:0000269|PubMed:11371182, ECO:0000269|PubMed:14275142, ECO:0000269|PubMed:19610625}; |
| Catalytic activity: | |
Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH; Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636, ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.20; Evidence={ECO:0000269|PubMed:11371182, ECO:0000269|PubMed:14275142, ECO:0000269|PubMed:19610625}; |
| Cofactor: | |
Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:10201405, ECO:0000269|PubMed:11371182, ECO:0000269|PubMed:14275142, ECO:0000269|PubMed:16114881, ECO:0000269|PubMed:16605249, ECO:0000269|PubMed:19610625}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=17 uM for NADPH {ECO:0000269|PubMed:10201405, ECO:0000269|PubMed:14275142}; KM=3.9 uM for 5,10-methylenetetrahydrofolate {ECO:0000269|PubMed:10201405, ECO:0000269|PubMed:14275142}; pH dependence: Optimum pH is 6.3-6.4. {ECO:0000269|PubMed:10201405, ECO:0000269|PubMed:14275142}; |
| Pathway: | |
One-carbon metabolism; tetrahydrofolate interconversion. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:10201405, ECO:0000269|PubMed:16114881, ECO:0000269|PubMed:16605249}. |
| Induction: | |
Repressed by methionine. {ECO:0000269|PubMed:14275142}. |
| Similarity: | |
Belongs to the methylenetetrahydrofolate reductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.