UniProt functional annotation for P04693

UniProt code: P04693.

Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.
Function:		Broad-specificity enzyme that catalyzes the transamination of 2-ketoisocaproate, p-hydroxyphenylpyruvate, and phenylpyruvate to yield leucine, tyrosine, and phenylalanine, respectively. In vitro, is able to catalyze the conversion of beta-methyl phenylpyruvate to the nonproteinogenic amino acid (2S,3S)-beta-methyl-phenylalanine, a building block of the antibiotic mannopeptimycin produced by Streptomyces hygroscopicus NRRL3085. {ECO:0000269|PubMed:19731276}.
Catalytic activity:		Reaction=2-oxoglutarate + an aromatic L-alpha-amino acid = an aromatic oxo-acid + L-glutamate; Xref=Rhea:RHEA:17533, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:73309, ChEBI:CHEBI:84824; EC=2.6.1.57; Evidence={ECO:0000269|PubMed:19731276};
Catalytic activity:		Reaction=(2S,3S)-3-methylphenylalanine + 2-oxoglutarate = (3S)-2-oxo-3- phenylbutanoate + L-glutamate; Xref=Rhea:RHEA:39911, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:74119, ChEBI:CHEBI:76864; EC=2.6.1.107; Evidence={ECO:0000269|PubMed:19731276};
Cofactor:		Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
Pathway:		Amino-acid biosynthesis; L-phenylalanine biosynthesis; L- phenylalanine from phenylpyruvate (ArAT route): step 1/1.
Pathway:		Amino-acid biosynthesis; L-tyrosine biosynthesis; L-tyrosine from (4-hydroxyphenyl)pyruvate: step 1/1.
Subunit:		Homodimer.
Subcellular location:		Cytoplasm.
Similarity:		Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.