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PDBsum entry 3fqq
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Metal binding protein
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PDB id
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3fqq
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References listed in PDB file
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Key reference
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Title
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Crystal structure of a novel dimeric form of ns5a domain i protein from hepatitis c virus.
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Authors
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R.A.Love,
O.Brodsky,
M.J.Hickey,
P.A.Wells,
C.N.Cronin.
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Ref.
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J Virol, 2009,
83,
4395-4403.
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PubMed id
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Abstract
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A new protein expression vector design utilizing an N-terminal six-histidine tag
and tobacco etch virus protease cleavage site upstream of the hepatitis C virus
NS5A sequence has resulted in a more straightforward purification method and
improved yields of purified NS5A domain I protein. High-resolution diffracting
crystals of NS5A domain I (amino acids 33 to 202) [NS5A(33-202)] were obtained
by using detergent additive crystallization screens, leading to the structure of
a homodimer which is organized differently from that published previously (T. L.
Tellinghuisen, J. Marcotrigiano, and C. M. Rice, Nature 435:374-379, 2005) yet
is consistent with a membrane association model for NS5A. The monomer-monomer
interface of NS5A(33-202) features an extensive buried surface area involving
the most-highly conserved face of each monomer. The two alternate structural
forms of domain I now available may be indicative of the multiple roles emerging
for NS5A in viral RNA replication and viral particle assembly.
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