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UniProt functional annotation for Q81G39 | ||
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| UniProt code: Q81G39. |
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| Organism: | |
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group. |
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| Function: | |
Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D- alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall. {ECO:0000255|HAMAP-Rule:MF_00593, ECO:0000269|PubMed:18847223}. |
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| Catalytic activity: | |
Reaction=ATP + D-alanine + holo-[D-alanyl-carrier protein] = AMP + D- alanyl-[D-alanyl-carrier protein] + diphosphate; Xref=Rhea:RHEA:55132, Rhea:RHEA-COMP:14102, Rhea:RHEA-COMP:14103, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57416, ChEBI:CHEBI:64479, ChEBI:CHEBI:138620, ChEBI:CHEBI:456215; EC=6.2.1.54; Evidence={ECO:0000255|HAMAP-Rule:MF_00593, ECO:0000269|PubMed:18847223}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.1 mM for D-alanine {ECO:0000269|PubMed:18847223}; KM=14.4 mM for L-alanine {ECO:0000269|PubMed:18847223}; |
| Pathway: | |
Cell wall biogenesis; lipoteichoic acid biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00593}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00593}. |
| Similarity: | |
Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily. {ECO:0000255|HAMAP-Rule:MF_00593}. |
Annotations taken from UniProtKB at the EBI.