|
|
||
|
|
|
|
|
UniProt functional annotation for A5JUY8 | ||
|
|
|
|
|
|
||
| UniProt code: A5JUY8. |
|
||
| Organism: | |
Bubalus bubalis (Domestic water buffalo). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bubalus. |
|
||
|
||
| Function: | |
Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). May protect the udder from infection and promote growth in newborn calves. Inhibits growth of the following bacterial species: E.coli, K.pneumoniae, P.aeruginosa, S.sonnei, S.saphrophyticus, S.epidermidis, and S.dysenteriae. {ECO:0000269|PubMed:12071645}. |
|
||
| Catalytic activity: | |
Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7; Evidence={ECO:0000269|PubMed:12071645, ECO:0000269|PubMed:19339248}; |
| Cofactor: | |
Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000255|PROSITE-ProRule:PRU00298, ECO:0000269|PubMed:19339248}; Note=Binds 1 Ca(2+) ion per heterodimer. {ECO:0000255|PROSITE- ProRule:PRU00298, ECO:0000269|PubMed:19339248}; |
| Cofactor: | |
Name=heme b; Xref=ChEBI:CHEBI:60344; Evidence={ECO:0000255|PROSITE-ProRule:PRU00298, ECO:0000269|PubMed:19339248}; Note=Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per heterodimer. {ECO:0000255|PROSITE-ProRule:PRU00298, ECO:0000269|PubMed:19339248}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.82 mM for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (at 60 degrees Celsius) {ECO:0000269|PubMed:12071645}; KM=0.77 mM for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (at 25 degrees Celsius) {ECO:0000269|PubMed:12071645}; pH dependence: Optimum pH is 6. {ECO:0000269|PubMed:12071645}; Temperature dependence: Optimum temperature is 60 degrees Celsius. {ECO:0000269|PubMed:12071645}; |
| Subcellular location: | |
Secreted {ECO:0000269|PubMed:12071645, ECO:0000269|PubMed:19339248, ECO:0000269|PubMed:30296068}. |
| Tissue specificity: | |
Mammary gland; milk. {ECO:0000269|PubMed:12071645, ECO:0000269|PubMed:19339248, ECO:0000269|PubMed:30296068}. |
| Similarity: | |
Belongs to the peroxidase family. XPO subfamily. {ECO:0000255|PROSITE-ProRule:PRU00298}. |
Annotations taken from UniProtKB at the EBI.