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PDBsum entry 3f2e
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Viral protein
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PDB id
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3f2e
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References listed in PDB file
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Key reference
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Title
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Synergy of nmr, Computation, And X-Ray crystallography for structural biology.
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Authors
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B.R.Szymczyna,
R.E.Taurog,
M.J.Young,
J.C.Snyder,
J.E.Johnson,
J.R.Williamson.
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Ref.
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Structure, 2009,
17,
499-507.
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PubMed id
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Abstract
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NMR spectroscopy and X-ray crystallography are currently the two most widely
applied methods for the determination of macromolecular structures at high
resolution. More recently, significant advances have been made in algorithms for
the de novo prediction of protein structure, and, in favorable cases, the
predicted models agree extremely well with experimentally determined structures.
Here, we demonstrate a synergistic combination of NMR spectroscopy, de novo
structure prediction, and X-ray crystallography in an effective overall strategy
for rapidly determining the structure of the coat protein C-terminal domain from
the Sulfolobus islandicus rod-shaped virus (SIRV). This approach takes advantage
of the most accessible aspects of each structural technique and may be widely
applicable for structure determination.
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