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UniProt functional annotation for Q5EK40 | ||
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| UniProt code: Q5EK40. |
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| Organism: | |
Vibrio cholerae. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio. |
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| Function: | |
An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor- related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae. {ECO:0000269|PubMed:18276581, ECO:0000269|PubMed:19793133}. |
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| Catalytic activity: | |
Reaction=diphthamide-[translation elongation factor 2] + NAD(+) = H(+) + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2] + nicotinamide; Xref=Rhea:RHEA:11820, Rhea:RHEA-COMP:10174, Rhea:RHEA- COMP:10175, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:82697; EC=2.4.2.36; |
| Activity regulation: | |
Partially inhibited by 1,8-naphthalimide (NAP). {ECO:0000269|PubMed:19793133}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=45 uM for NAD {ECO:0000269|PubMed:18276581}; Note=For ADP-ribosyltransferase activity of a catalytic fragment of residues 459-666.; |
| Ptm: | |
Probably requires further proteolytic processing to generate a fragment with toxic activity. |
| Miscellaneous: | |
Exotoxins are often highly strain specific; not encoded in strain ATCC 39315 / El Tor Inabe N16961. |
Annotations taken from UniProtKB at the EBI.