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UniProt functional annotation for Q5UQL3 | ||
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| UniProt code: Q5UQL3. |
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| Organism: | |
Acanthamoeba polyphaga mimivirus (APMV). |
| Taxonomy: | |
Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Imitervirales; Mimiviridae; Mimivirus. |
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| Function: | |
Plays a role in the synthesis of nucleoside triphosphates. This activity may optimize the replication of the AT-rich (73%) viral genome in a thymidine-limited host environment. |
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| Catalytic activity: | |
Reaction=a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'- deoxyribonucleoside 5'-triphosphate + ADP; Xref=Rhea:RHEA:44640, ChEBI:CHEBI:30616, ChEBI:CHEBI:61560, ChEBI:CHEBI:73316, ChEBI:CHEBI:456216; EC=2.7.4.6; Evidence={ECO:0000255|PROSITE- ProRule:PRU10030}; |
| Catalytic activity: | |
Reaction=a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'- triphosphate + ADP; Xref=Rhea:RHEA:18113, ChEBI:CHEBI:30616, ChEBI:CHEBI:57930, ChEBI:CHEBI:61557, ChEBI:CHEBI:456216; EC=2.7.4.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10030}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=140 uM for dGDP {ECO:0000269|PubMed:16957983}; KM=41 uM for dGTP {ECO:0000269|PubMed:16957983}; KM=5 uM for ADP {ECO:0000269|PubMed:16957983}; KM=770 uM for ATP {ECO:0000269|PubMed:16957983}; KM=830 uM for GTP {ECO:0000269|PubMed:16957983}; KM=100 uM for UDP {ECO:0000269|PubMed:16957983}; KM=110 uM for UTP {ECO:0000269|PubMed:16957983}; KM=40 uM for CTP {ECO:0000269|PubMed:16957983}; KM=50 uM for CDP {ECO:0000269|PubMed:16957983}; KM=30 uM for dTTP {ECO:0000269|PubMed:16957983}; KM=25 uM for dUTP {ECO:0000269|PubMed:16957983}; Vmax=99 umol/min/mg enzyme toward dGDP {ECO:0000269|PubMed:16957983}; Vmax=27 umol/min/mg enzyme toward dGTP {ECO:0000269|PubMed:16957983}; Vmax=50 umol/min/mg enzyme toward ADP {ECO:0000269|PubMed:16957983}; Vmax=140 umol/min/mg enzyme toward ATP {ECO:0000269|PubMed:16957983}; Vmax=450 umol/min/mg enzyme toward GTP {ECO:0000269|PubMed:16957983}; Vmax=110 umol/min/mg enzyme toward UDP {ECO:0000269|PubMed:16957983}; Vmax=41 umol/min/mg enzyme toward UTP {ECO:0000269|PubMed:16957983}; Vmax=25 umol/min/mg enzyme toward CTP {ECO:0000269|PubMed:16957983}; Vmax=70 umol/min/mg enzyme toward CDP {ECO:0000269|PubMed:16957983}; Note=KM values are below 10 uM for dCDP, dCTP and dUTP.; |
| Similarity: | |
Belongs to the NDK family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.