|
|
||
|
|
|
|
|
UniProt functional annotation for P53554 | ||
|
|
|
|
|
|
||
| UniProt code: P53554. |
|
||
| Organism: | |
Bacillus subtilis (strain 168). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. |
|
||
|
||
| Function: | |
Catalyzes the C-C bond cleavage of fatty acid linked to acyl carrier protein (ACP) to generate pimelic acid for biotin biosynthesis. It has high affinity for long-chain fatty acids with the greatest affinity for myristic acid. {ECO:0000269|PubMed:11368323, ECO:0000269|PubMed:8763940}. |
|
||
| Catalytic activity: | |
Reaction=a C2-C8-saturated long-chain fatty acyl-[ACP] + 3 O2 + 2 reduced [flavodoxin] = 6-carboxyhexanoyl-[ACP] + a fatty aldehyde + 3 H(+) + 3 H2O + 2 oxidized [flavodoxin]; Xref=Rhea:RHEA:52852, Rhea:RHEA-COMP:9955, Rhea:RHEA-COMP:10622, Rhea:RHEA-COMP:10623, Rhea:RHEA-COMP:13382, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:35746, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:78846, ChEBI:CHEBI:136878; EC=1.14.14.46; Evidence={ECO:0000269|PubMed:11368323}; |
| Cofactor: | |
Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000269|PubMed:18838690}; Note=Binds 1 heme group covalently per subunit. {ECO:0000269|PubMed:18838690}; |
| Pathway: | |
Cofactor biosynthesis; biotin biosynthesis. |
| Mass spectrometry: | |
Mass=44732; Method=Electrospray; Evidence={ECO:0000269|PubMed:11472016}; |
| Mass spectrometry: | |
Mass=45348; Method=Electrospray; Evidence={ECO:0000269|PubMed:11368323}; |
| Similarity: | |
Belongs to the cytochrome P450 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.