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UniProt functional annotation for P11509 | ||
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| UniProt code: P11509. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo- monooxygenase. Possesses low phenacetin O-deethylation activity. {ECO:0000269|PubMed:11695850, ECO:0000269|PubMed:16086027, ECO:0000269|PubMed:17125252, ECO:0000269|PubMed:18779312, ECO:0000269|PubMed:1889415, ECO:0000269|PubMed:1944238}. |
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| Catalytic activity: | |
Reaction=1,4-cineole + O2 + reduced [NADPH--hemoprotein reductase] = 2- exo-hydroxy-1,4-cineole + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:49160, Rhea:RHEA-COMP:11964, Rhea:RHEA- COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:80788, ChEBI:CHEBI:90956; Evidence={ECO:0000269|PubMed:11695850}; |
| Cofactor: | |
Name=heme; Xref=ChEBI:CHEBI:30413; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.23 uM for coumarin {ECO:0000269|PubMed:16086027}; KM=530 uM for 1,4-cineole {ECO:0000269|PubMed:11695850}; Vmax=3.5 nmol/min/nmol enzyme toward 1,4-cineole {ECO:0000269|PubMed:11695850}; |
| Subcellular location: | |
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Tissue specificity: | |
Liver. {ECO:0000269|PubMed:1889415, ECO:0000269|PubMed:1944238}. |
| Induction: | |
By phenobarbital and dexamethasone. {ECO:0000269|PubMed:1889415, ECO:0000269|PubMed:1944238}. |
| Similarity: | |
Belongs to the cytochrome P450 family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAA52147.1; Type=Miscellaneous discrepancy; Note=Numerous conflicts and frameshifts.; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.