PDBsum entry 3dow

Protein transport

PDB id: 3dow

Contents

Protein chain

119 a.a.

Ligands

ASP-TRP-ASP-PHE-LEU-PRO-PRO-NH2

Metals

_ZN

Waters ×29

References listed in PDB file

Key reference

• Title: Structural framework of the gabarap-Calreticulin interface--Implications for substrate binding to endoplasmic reticulum chaperones.
• Authors: Y.Thielmann, O.H.Weiergräber, J.Mohrlüder, D.Willbold.
• Ref.: Febs J, 2009, 276, 1140-1152.
• PubMed id: 19154346

Abstract

The 4-aminobutyrate type A receptor-associated protein (GABARAP) is a versatile adaptor protein that plays an important role in intracellular vesicle trafficking, particularly in neuronal cells. We have investigated the structural determinants underlying the interaction of GABARAP with calreticulin using spectroscopic and crystallographic techniques. Specifically, we present the crystal structure of GABARAP in complex with its major binding epitope on the chaperone. Molecular modeling of a complex containing full-length calreticulin suggests a novel mode of substrate interaction, which may have functional implications for the calreticulin/calnexin family in general.