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UniProt functional annotation for P09211 | ||
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| UniProt code: P09211. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276). Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. {ECO:0000269|PubMed:21046276, ECO:0000269|PubMed:21668448, ECO:0000269|PubMed:9084911}. |
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| Catalytic activity: | |
Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; Evidence={ECO:0000269|PubMed:1540159, ECO:0000269|PubMed:1567427, ECO:0000269|PubMed:8433974}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16438; Evidence={ECO:0000305}; |
| Catalytic activity: | |
Reaction=glutathione + prostaglandin J2 = prostaglandin J2-S-(R)- glutathione; Xref=Rhea:RHEA:50804, ChEBI:CHEBI:57925, ChEBI:CHEBI:133396, ChEBI:CHEBI:133771; Evidence={ECO:0000269|PubMed:9084911}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50805; Evidence={ECO:0000305|PubMed:9084911}; |
| Catalytic activity: | |
Reaction=glutathione + prostaglandin J2 = prostaglandin J2-S-(S)- glutathione; Xref=Rhea:RHEA:50808, ChEBI:CHEBI:57925, ChEBI:CHEBI:133396, ChEBI:CHEBI:133772; Evidence={ECO:0000269|PubMed:9084911}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50809; Evidence={ECO:0000305|PubMed:9084911}; |
| Catalytic activity: | |
Reaction=glutathione + prostaglandin A2 = prostaglandin A2-S-(S)- glutathione; Xref=Rhea:RHEA:50800, ChEBI:CHEBI:57925, ChEBI:CHEBI:133370, ChEBI:CHEBI:133769; Evidence={ECO:0000269|PubMed:9084911}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50801; Evidence={ECO:0000305|PubMed:9084911}; |
| Catalytic activity: | |
Reaction=11(S)-hydroxy-14(S),15(S)-epoxy-(5Z,8Z,12E)-eicosatrienoate + glutathione = (11S,15S)-dihydroxy-14(R)-S-glutathionyl-(5Z,8Z,12E)- eicosatrienoate; Xref=Rhea:RHEA:50260, ChEBI:CHEBI:57925, ChEBI:CHEBI:132200, ChEBI:CHEBI:132201; Evidence={ECO:0000269|PubMed:21046276}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50261; Evidence={ECO:0000305|PubMed:21046276}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=395 uM for prostaglandin A2 (at pH 7.0 and 37 degrees Celsius) {ECO:0000269|PubMed:9084911}; KM=201 uM for prostaglandin J2 (at pH 7.0 and 37 degrees Celsius) {ECO:0000269|PubMed:9084911}; Vmax=267 nmol/min/mg enzyme for the formation of the glutathione-S- conjugate of prostaglandin A2 (at pH 7.0 and 37 degrees Celsius) {ECO:0000269|PubMed:9084911}; Vmax=105 nmol/min/mg enzyme for the formation of the glutathione-S- conjugate of prostaglandin J2 (at pH 7.0 and 37 degrees Celsius) {ECO:0000269|PubMed:9084911}; |
| Subunit: | |
Homodimer. Interacts with CDK5. {ECO:0000269|PubMed:21668448, ECO:0000269|PubMed:9012673}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:19269317}. Mitochondrion {ECO:0000269|PubMed:19269317}. Nucleus {ECO:0000269|PubMed:19269317}. Note=The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization. |
| Similarity: | |
Belongs to the GST superfamily. Pi family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.