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UniProt functional annotation for Q8TBC4 | ||
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| UniProt code: Q8TBC4. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down- regulates steroid receptor activity. Necessary for cell cycle progression. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:12740388, ECO:0000269|PubMed:9694792}. |
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| Catalytic activity: | |
[NEDD8-activating enzyme E1 catalytic subunit]: Reaction=ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L- cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.; EC=6.2.1.64; Evidence={ECO:0000269|PubMed:9694792}; |
| Activity regulation: | |
Binding of TP53BP2 to the regulatory subunit NAE1 decreases activity. |
| Pathway: | |
Protein modification; protein neddylation. |
| Subunit: | |
Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand (By similarity). Interacts with TBATA (By similarity). {ECO:0000250}. |
| Tissue specificity: | |
Ubiquitously expressed. {ECO:0000269|PubMed:10207026}. |
| Miscellaneous: | |
Arg-211 acts as a selectivity gate, preventing misactivation of ubiquitin by this NEDD8-specific E1 complex. |
| Similarity: | |
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAC27648.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.