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PDBsum entry 3dax
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Oxidoreductase
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PDB id
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3dax
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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Crystal structure of human cyp7a1
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Structure:
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Cytochrome p450 7a1. Chain: a, b. Synonym: cholesterol 7-alpha-monooxygenase, cypvii, cholesterol 7- alpha-hydroxylase. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: cyp7a1, cyp7. Expressed in: escherichia coli.
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Resolution:
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2.15Å
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R-factor:
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0.190
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R-free:
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0.228
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Authors:
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N.V.Strushkevich,W.Tempel,L.Dombrovski,A.Dong,P.Loppnau, C.H.Arrowsmith,A.M.Edwards,C.Bountra,M.Wilkstrom,A.Bochkarev,H.Park, Structural Genomics Consortium (Sgc)
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Key ref:
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N.V.Strushkevich
et al.
Crystal structure of human cyp7a1.
To be published,
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Date:
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30-May-08
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Release date:
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05-Aug-08
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PROCHECK
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Headers
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References
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P22680
(CP7A1_HUMAN) -
Cytochrome P450 7A1 from Homo sapiens
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Seq:
Struc:
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504 a.a.
472 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Enzyme class 1:
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E.C.1.14.14.23
- cholesterol 7alpha-monooxygenase.
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Reaction:
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cholesterol + reduced [NADPH--hemoprotein reductase] + O2 = 7alpha- hydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H+
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cholesterol
Bound ligand (Het Group name = )
matches with 61.36% similarity
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+
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reduced [NADPH--hemoprotein reductase]
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+
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O2
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=
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7alpha- hydroxycholesterol
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+
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oxidized [NADPH--hemoprotein reductase]
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+
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H2O
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+
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H(+)
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Cofactor:
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Heme-thiolate
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Enzyme class 2:
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E.C.1.14.14.26
- 24-hydroxycholesterol 7alpha-hydroxylase.
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Reaction:
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(24S)-hydroxycholesterol + reduced [NADPH--hemoprotein reductase] + O2 = (24S)-7alpha-dihydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H+
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(24S)-hydroxycholesterol
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reduced [NADPH--hemoprotein reductase]
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+
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O2
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=
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(24S)-7alpha-dihydroxycholesterol
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+
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oxidized [NADPH--hemoprotein reductase]
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+
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H2O
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+
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H(+)
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Cofactor:
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Heme-thiolate
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
