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UniProt functional annotation for P30986 | ||
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| UniProt code: P30986. |
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| Organism: | |
Eschscholzia californica (California poppy). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Ranunculales; Papaveraceae; Eschscholzioideae; Eschscholzia. |
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| Function: | |
Essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Catalyzes the stereospecific conversion of the N-methyl moiety of (S)-reticuline into the berberine bridge carbon of (S)-scoulerine. |
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| Catalytic activity: | |
Reaction=(S)-reticuline + O2 = (S)-scoulerine + H(+) + H2O2; Xref=Rhea:RHEA:19885, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:17129, ChEBI:CHEBI:57873; EC=1.21.3.3; |
| Cofactor: | |
Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:16728404, ECO:0000269|PubMed:19457868}; Note=Binds 1 FAD per subunit in a bicovalent manner. {ECO:0000269|PubMed:16728404, ECO:0000269|PubMed:19457868}; |
| Cofactor: | |
Name=a metal cation; Xref=ChEBI:CHEBI:25213; |
| Pathway: | |
Alkaloid biosynthesis; (S)-scoulerine biosynthesis; (S)- scoulerine from (S)-reticuline: step 1/1. |
| Subcellular location: | |
Cytoplasmic vesicle {ECO:0000269|PubMed:24240298}. Note=Found in cytoplasmic vesicles that are highly specific and unique compartments serving only alkaloid biosynthesis. The protein composition of these vesicles reveals the presence of only about 20 separable proteins. {ECO:0000269|PubMed:24240298}. |
| Ptm: | |
The FAD cofactor is bound via a bicovalent 6-S-cysteinyl, 8alpha- N1-histidyl FAD linkage. {ECO:0000269|PubMed:16728404, ECO:0000269|PubMed:19457868}. |
| Similarity: | |
Belongs to the oxygen-dependent FAD-linked oxidoreductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.