|
|
||
|
|
|
|
|
UniProt functional annotation for Q83RJ4 | ||
|
|
|
|
|
|
||
| UniProt code: Q83RJ4. |
|
||
| Organism: | |
Shigella flexneri. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Shigella. |
|
||
|
||
| Function: | |
Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway. Synthesizes a 'Lys-48'-linked ubiquitin chain, which requires non- covalent binding between ubiquitin and the host ubiquitin-conjugating enzyme UBE2D1. {ECO:0000269|PubMed:18997779}. |
|
||
| Catalytic activity: | |
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; |
| Subcellular location: | |
Secreted {ECO:0000250}. Host cytoplasm {ECO:0000250}. Note=Secreted via Mxi-Spa type III secretion system (TTSS), and delivered into the host cytoplasm. {ECO:0000250}. |
| Domain: | |
The LRR (leucine-rich repeat) domain forms a slightly curved solenoid and may mediate interaction with target proteins. {ECO:0000269|PubMed:18997779}. |
| Ptm: | |
Ubiquitinated in the presence of host E1 ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzyme UBE2D3 and ubiquitin. {ECO:0000269|PubMed:18997779}. |
| Similarity: | |
Belongs to the LRR-containing bacterial E3 ligase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.