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PDBsum entry 3cpp
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Oxidoreductase(oxygenase)
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PDB id
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3cpp
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the carbon monoxide-Substrate-Cytochrome p-450cam ternary complex.
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Authors
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R.Raag,
T.L.Poulos.
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Ref.
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Biochemistry, 1989,
28,
7586-7592.
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PubMed id
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Abstract
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The crystal structure of the ternary complex formed between carbon monoxide
(CO), camphor, and ferrous cytochrome P-450CAM has been refined to an R value of
17.9% at 1.9-A resolution. To accommodate the CO molecule, the substrate,
camphor, moves about 0.8 A while at the same time remaining in nonbonded contact
with CO. The average temperature factor of the camphor atoms is about 50% higher
in the CO complex, suggesting that the camphor is more loosely bound in this
ternary complex. The Fe-C-O angle is about 166 degrees, and thus, CO appears to
be bent from the heme normal, as it is in various CO-globin complexes, due to
steric interactions with active site groups. The oxygen atom of the CO molecule
is nestled into a groove formed by an unusual helical hydrogen bond in the
distal helix between the highly conserved Thr 252 and Gly 248 residues. In the
transition from the ferric camphor-bound binary complex to the ferrous
CO-camphor-bound ternary complex, the heme iron atom moves into the plane
defined by the pyrrole nitrogens by about 0.41 A. Although the axial Cys ligand
also moves toward the heme, the S-Fe bond stretches from about 2.20 A in the
absence of CO to about 2.41 A once CO has bound.
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Secondary reference #1
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Title
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The structural basis for substrate-Induced changes in redox potential and spin equilibrium in cytochrome p-450cam.
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Authors
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R.Raag,
T.L.Poulos.
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Ref.
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Biochemistry, 1989,
28,
917-922.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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High-Resolution crystal structure of cytochrome p450cam.
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Authors
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T.L.Poulos,
B.C.Finzel,
A.J.Howard.
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Ref.
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J Mol Biol, 1987,
195,
687-700.
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PubMed id
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Secondary reference #3
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Title
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The 2.6-A crystal structure of pseudomonas putida cytochrome p-450.
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Authors
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T.L.Poulos,
B.C.Finzel,
I.C.Gunsalus,
G.C.Wagner,
J.Kraut.
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Ref.
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J Biol Chem, 1985,
260,
16122-16130.
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PubMed id
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Secondary reference #4
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Title
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Crystal structure of substrate-Free pseudomonas putida cytochrome p-450.
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Authors
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T.L.Poulos,
B.C.Finzel,
A.J.Howard.
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Ref.
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Biochemistry, 1986,
25,
5314-5322.
[DOI no: ]
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PubMed id
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Secondary reference #5
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Title
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Heme enzyme structure and function
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Authors
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T.L.Poulos,
B.C.Finzel.
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Ref.
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pept protein rev, 1984,
4,
115.
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Secondary reference #6
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Title
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Preliminary crystallographic data on cytochrome p-450cam.
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Authors
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T.L.Poulos,
M.Perez,
G.C.Wagner.
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Ref.
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J Biol Chem, 1982,
257,
10427-10429.
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PubMed id
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Secondary reference #7
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Title
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The primary structure of the monoxygenase cytochrome p450cam.
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Authors
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M.Haniu,
L.G.Armes,
M.Tanaka,
K.T.Yasunobu,
B.S.Shastry,
G.C.Wagner,
I.C.Gunsalus.
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Ref.
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Biochem Biophys Res Commun, 1982,
105,
889-894.
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PubMed id
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