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UniProt functional annotation for Q9FUZ2 | ||
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| UniProt code: Q9FUZ2. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
Removes the formyl group from the N-terminal Met of newly synthesized proteins. Has a preferred substrate specificity towards the photosystem II (PS II) D1 polypeptide. {ECO:0000269|PubMed:11060042}. |
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| Catalytic activity: | |
Reaction=H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N- terminal L-methionyl-[peptide]; Xref=Rhea:RHEA:24420, Rhea:RHEA- COMP:10639, Rhea:RHEA-COMP:10640, ChEBI:CHEBI:15377, ChEBI:CHEBI:15740, ChEBI:CHEBI:49298, ChEBI:CHEBI:64731; EC=3.5.1.88; |
| Cofactor: | |
Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000269|PubMed:11733990}; Note=Binds 1 Fe(2+) ion. {ECO:0000269|PubMed:11733990}; |
| Activity regulation: | |
Inhibited by actinonin. {ECO:0000269|PubMed:11553738, ECO:0000269|PubMed:11733990}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=130 uM for N-formyl-Met-Leu-rho-nitroanilide {ECO:0000269|PubMed:11553738}; KM=3200 uM for N-formyl-Met-Ala-Ser {ECO:0000269|PubMed:11553738}; Vmax=20 umol/min/mg enzyme toward N-formyl-Met-Leu-rho-nitroanilide {ECO:0000269|PubMed:11553738}; Vmax=1.3 umol/min/mg enzyme toward N-formyl-Met-Ala-Ser {ECO:0000269|PubMed:11553738}; |
| Subunit: | |
Homodimer. |
| Subcellular location: | |
Plastid, chloroplast stroma. Mitochondrion. Note=Reported to be localized to chloroplast and mitochondria based on transient GFP expression in a heterologous system. {ECO:0000269|PubMed:11060042}. |
| Tissue specificity: | |
Expressed in leaves and flowers. {ECO:0000269|PubMed:11060042}. |
| Disruption phenotype: | |
Albino. {ECO:0000269|PubMed:12505980}. |
| Similarity: | |
Belongs to the polypeptide deformylase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.