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PDBsum entry 3c0t
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Transcription
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PDB id
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3c0t
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References listed in PDB file
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Key reference
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Title
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Structure-System correlation identifies a gene regulatory mediator submodule.
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Authors
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L.Larivière,
M.Seizl,
S.Van wageningen,
S.Röther,
L.Van de pasch,
H.Feldmann,
K.Strässer,
S.Hahn,
F.C.Holstege,
P.Cramer.
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Ref.
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Genes Dev, 2008,
22,
872-877.
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PubMed id
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Abstract
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A combination of crystallography, biochemistry, and gene expression analysis
identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct
submodule of the Mediator head module. Med8C forms a conserved alpha-helix that
tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in
dissociation of Med18/20 from Mediator and in loss of transcription activity of
extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast
transcriptome, establishing Med8C/18/20 as a predominantly positive,
gene-specific submodule required for low transcription levels of nonactivated
genes, including conjugation genes. The presented structure-based system
perturbation is superior to gene deletion analysis of gene regulation.
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