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PDBsum entry 3c0m
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References listed in PDB file
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Key reference
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Title
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Molecular assembly of the aerolysin pore reveals a swirling membrane-Insertion mechanism.
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Authors
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M.T.Degiacomi,
I.Iacovache,
L.Pernot,
M.Chami,
M.Kudryashev,
H.Stahlberg,
F.G.Van der goot,
M.Dal peraro.
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Ref.
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Nat Chem Biol, 2013,
9,
623-629.
[DOI no: ]
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PubMed id
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Abstract
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Aerolysin is the founding member of a superfamily of β-pore-forming toxins
whose pore structure is unknown. We have combined X-ray crystallography,
cryo-EM, molecular dynamics and computational modeling to determine the
structures of aerolysin mutants in their monomeric and heptameric forms, trapped
at various stages of the pore formation process. A dynamic modeling approach
based on swarm intelligence was applied, whereby the intrinsic flexibility of
aerolysin extracted from new X-ray structures was used to fully exploit the
cryo-EM spatial restraints. Using this integrated strategy, we obtained a
radically new arrangement of the prepore conformation and a near-atomistic
structure of the aerolysin pore, which is fully consistent with all of the
biochemical data available so far. Upon transition from the prepore to pore, the
aerolysin heptamer shows a unique concerted swirling movement, accompanied by a
vertical collapse of the complex, ultimately leading to the insertion of a
transmembrane β-barrel.
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Secondary reference #1
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Title
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Structure of the aeromonas toxin proaerolysin in its water-Soluble and membrane-Channel states.
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Authors
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M.W.Parker,
J.T.Buckley,
J.P.Postma,
A.D.Tucker,
K.Leonard,
F.Pattus,
D.Tsernoglou.
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Ref.
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Nature, 1994,
367,
292-295.
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PubMed id
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Secondary reference #2
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Title
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Crystallization of a proform of aerolysin, A hole-Forming toxin from aeromonas hydrophila.
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Authors
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A.D.Tucker,
M.W.Parker,
D.Tsernoglou,
J.T.Buckley.
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Ref.
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J Mol Biol, 1990,
212,
561-562.
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PubMed id
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