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UniProt functional annotation for P9WPY3 | ||
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| UniProt code: P9WPY3. |
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| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
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| Function: | |
Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. {ECO:0000269|PubMed:11483005, ECO:0000269|PubMed:17020768}. |
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| Catalytic activity: | |
Reaction=ATP + shikimate = 3-phosphoshikimate + ADP + H(+); Xref=Rhea:RHEA:13121, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:36208, ChEBI:CHEBI:145989, ChEBI:CHEBI:456216; EC=2.7.1.71; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:12054870, ECO:0000269|PubMed:15583379}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000269|PubMed:12054870, ECO:0000269|PubMed:15583379}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=410 uM for shikimate {ECO:0000269|PubMed:12054870}; KM=83 uM for ATP {ECO:0000269|PubMed:12054870}; |
| Pathway: | |
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:11483005, ECO:0000269|PubMed:12054870, ECO:0000269|PubMed:15358538, ECO:0000269|PubMed:15583379, ECO:0000269|PubMed:16834327, ECO:0000269|PubMed:17020768, ECO:0000269|PubMed:17183161}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
| Domain: | |
Consists of three domains: the CORE domain which forms the binding site for nucleotides, the LID domain which closes over the active site upon ATP or shikimate binding, and the substrate-binding domain which functions to recognize and bind shikimate. |
| Miscellaneous: | |
The phosphoryl transfer proceeds by an in-line associative mechanism. The random sequential binding of shikimate and nucleotide is associated with domain movements that suggest a synergic mechanism by which binding of the first substrate may enhance the affinity for the second substrate. |
| Miscellaneous: | |
Was identified as a high-confidence drug target. |
| Similarity: | |
Belongs to the shikimate kinase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.