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UniProt functional annotation for P61615 | ||
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| UniProt code: P61615. |
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| Organism: | |
Saccharolobus shibatae (Sulfolobus shibatae). |
| Taxonomy: | |
Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Saccharolobus. |
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| Function: | |
Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP). {ECO:0000255|HAMAP-Rule:MF_00354, ECO:0000269|PubMed:19158086, ECO:0000269|PubMed:22158896, ECO:0000269|PubMed:22505674}. |
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| Catalytic activity: | |
Reaction=isopentenyl diphosphate = dimethylallyl diphosphate; Xref=Rhea:RHEA:23284, ChEBI:CHEBI:57623, ChEBI:CHEBI:128769; EC=5.3.3.2; Evidence={ECO:0000255|HAMAP-Rule:MF_00354, ECO:0000269|PubMed:22158896}; |
| Cofactor: | |
Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:19158086, ECO:0000269|PubMed:22158896, ECO:0000269|PubMed:22505674}; |
| Cofactor: | |
Name=NADPH; Xref=ChEBI:CHEBI:57783; Evidence={ECO:0000269|PubMed:19158086}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:19158086, ECO:0000269|PubMed:22158896}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=7.39 uM for IPP (at pH 6 and 60 degrees Celsius) {ECO:0000269|PubMed:22158896}; Note=Kcat is 29.9 sec(-1) for isomerase activity with IPP (at pH 6 and 60 degrees Celsius).; |
| Subunit: | |
Homooctamer. Dimer of tetramers. {ECO:0000255|HAMAP- Rule:MF_00354, ECO:0000269|PubMed:19158086, ECO:0000269|PubMed:22158896, ECO:0000269|PubMed:22505674}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00354}. |
| Similarity: | |
Belongs to the IPP isomerase type 2 family. {ECO:0000255|HAMAP-Rule:MF_00354}. |
Annotations taken from UniProtKB at the EBI.