PDBsum entry 3acf

Hydrolase

PDB id: 3acf

Contents

Protein chain

193 a.a.

Ligands

SO4

Metals

_CA

Waters ×322

References listed in PDB file

Key reference

• Title: Recognition of cellooligosaccharides by a family 28 carbohydrate-Binding module.
• Authors: K.Tsukimoto, R.Takada, Y.Araki, K.Suzuki, S.Karita, T.Wakagi, H.Shoun, T.Watanabe, S.Fushinobu.
• Ref.: Febs Lett, 2010, 584, 1205-1211.
• PubMed id: 20159017

Abstract

The crystal structures of a carbohydrate-binding module (CBM) family 28 domain of endoglucanase Cel5A from Clostridium josui have been determined in ligand-free and complex forms with cellobiose, cellotetraose, and cellopentaose as the first complex structures of this family. In the cleft of a beta-sandwich fold, the ligands are recognized by stacking interactions and hydrogen bonds. Conformations of the bound cellooligosaccharides are similar to those in crystals and solution but clearly different from the cellulose structure. Interestingly, the glucan chain bound on CBM28 is in the opposite direction of that bound to CBM17, although these families share significant structural similarity.