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UniProt functional annotation for P13448 | ||
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| UniProt code: P13448. |
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| Organism: | |
Rhodococcus erythropolis (Arthrobacter picolinophilus). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus. |
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| Function: | |
NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class. |
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| Catalytic activity: | |
Reaction=an aliphatic amide = a nitrile + H2O; Xref=Rhea:RHEA:12673, ChEBI:CHEBI:15377, ChEBI:CHEBI:18379, ChEBI:CHEBI:65285; EC=4.2.1.84; |
| Cofactor: | |
Name=Fe(3+); Xref=ChEBI:CHEBI:29034; Evidence={ECO:0000269|PubMed:9586994}; Note=Binds 1 Fe(3+) ion per subunit. {ECO:0000269|PubMed:9586994}; |
| Activity regulation: | |
Inactivated by nitrosylation of the iron center in the dark and activated by photo-induced nitric oxide (NO) release. Inactivated by oxidation of Cys-115 to a sulfenic acid. |
| Subunit: | |
Heterodimer of an alpha and a beta chain. |
| Ptm: | |
Oxidation on Cys-113 is essential for the activity. {ECO:0000269|PubMed:9368004, ECO:0000269|PubMed:9586994}. |
| Ptm: | |
Oxidation on Cys-115 stabilizes the Fe-NO ligand coordinated in the inactive form. {ECO:0000269|PubMed:9586994}. |
| Similarity: | |
Belongs to the nitrile hydratase subunit alpha family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.