PDBsum entry 3a5m

Contractile protein

PDB id: 3a5m

Contents

Protein chains

123 a.a. *

365 a.a. *

Ligands

ATP

Metals

_CA ×2

_MG

Waters ×358

* Residue conservation analysis

PDB id:

3a5m

Name:

Contractile protein

Title:

Crystal structure of a dictyostelium p109i mg2+-actin in complex with human gelsolin segment 1

Structure:

Gelsolin. Chain: s. Fragment: gelsolin-like 1, residues 53-176. Synonym: actin-depolymerizing factor, adf, brevin, agel. Engineered: yes. Mutation: yes. Major actin. Chain: c. Synonym: actin-1, actin-2, actin-2-sub 1, actin-4, actin-5, actin-6,

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Dictyostelium discoideum. Slime mold. Organism_taxid: 44689. Expressed in: dictyostelium.

Resolution:

2.40Å R-factor: 0.182 R-free: 0.223

Authors:

K.Murakami,T.Yasunaga,T.Q.Noguchi,T.Q.Uyeda,T.Wakabayashi

Key ref:

K.Murakami et al. (2010). Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release. Cell, 143, 275-287. PubMed id: 20946985

Date:

09-Aug-09 Release date: 27-Oct-10

Protein chain

P06396  (GELS_HUMAN) -  Gelsolin from Homo sapiens

Seq: 782 a.a.

Struc: 123 a.a.*

Protein chain

P07830  (ACT1_DICDI) -  Major actin from Dictyostelium discoideum

Seq: 376 a.a.

Struc: 365 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain C: E.C.3.6.4.- - ?????

Cell 143:275-287 (2010)

PubMed id: 20946985

Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.

K.Murakami, T.Yasunaga, T.Q.Noguchi, Y.Gomibuchi, K.X.Ngo, T.Q.Uyeda, T.Wakabayashi.

ABSTRACT

No abstract given.