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UniProt functional annotation for C4B644 | ||
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| UniProt code: C4B644. |
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| Organism: | |
Pseudonocardia autotrophica (Amycolata autotrophica) (Nocardia autotrophica). |
| Taxonomy: | |
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia. |
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| Function: | |
Hydroxylates vitamin D(3) into 25-hydroxyvitamin D(3) and 1- alpha,25-dihydroxyvitamin D(3), its physiologically active forms. It first hydroxylates the C-25 position of vitamin D(3) to form 25- hydroxyvitamin D(3), then subsequently hydroxylates the C-1-alpha position to form 1-alpha,25-dihydroxyvitamin D(3). Also displays 25- hydroxylase activity on vitamin D(2) and 7-dehydrocholesterol. May play a role in the biosynthesis of steroid metabolic intermediates. {ECO:0000269|PubMed:19450562}. |
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| Catalytic activity: | |
Reaction=5beta-cholestane-3alpha,7alpha,12alpha-triol + 5 H(+) + 3 O2 + 6 reduced [adrenodoxin] = (25R)-3alpha,7alpha,12alpha-trihydroxy- 5beta-cholestan-26-oate + 4 H2O + 6 oxidized [adrenodoxin]; Xref=Rhea:RHEA:34631, Rhea:RHEA-COMP:9998, Rhea:RHEA-COMP:9999, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16496, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58734; EC=1.14.15.15; Evidence={ECO:0000269|PubMed:19450562}; |
| Cofactor: | |
Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250|UniProtKB:P0A512}; |
| Activity regulation: | |
Activated by partially methylated beta- cyclodextrin. {ECO:0000269|PubMed:19450562}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=13.5 uM for vitamin D(3) {ECO:0000269|PubMed:19450562}; KM=7.1 uM for 25-hydroxyvitamin D(3) {ECO:0000269|PubMed:19450562}; |
| Subcellular location: | |
Cytoplasm {ECO:0000250|UniProtKB:P0A512}. |
| Similarity: | |
Belongs to the cytochrome P450 family. {ECO:0000255}. |
Annotations taken from UniProtKB at the EBI.