UniProt functional annotation for Q9KB30



	UniProt functional annotation for Q9KB30

UniProt code: Q9KB30.

Organism: Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125).

Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.

Function: Hydrolyzes xylooligosaccharides with a degree of polymerization of greater than or equal to 3, releasing xylose from the reducing end. Only hydrolyzes the beta anomers of xylooligosaccharides, with inversion of anomeric configuration. Hydrolyzes the glucose and xylose-based trisaccharides where xylose is located at the -1 subsite, GXX, XXG and GXG. Does not hydrolyze xylan, chitosan, lichenan, curdlan or carboxymethylcellulose. {ECO:0000269|PubMed:15491996}.

Catalytic activity: Reaction=Hydrolysis of (1->4)-beta-D-xylose residues from the reducing end of oligosaccharides.; EC=3.2.1.156; Evidence={ECO:0000269|PubMed:15491996};

Biophysicochemical properties: Kinetic parameters: KM=2.4 mM for X3 (in 50 mM sodium phosphate, pH 7.1, 40 degrees Celsius) {ECO:0000269|PubMed:15491996, ECO:0000269|PubMed:16301312}; KM=5.0 mM for X4 (in 50 mM sodium phosphate, pH 7.1, 40 degrees Celsius) {ECO:0000269|PubMed:15491996, ECO:0000269|PubMed:16301312}; KM=4.4 mM for X5 (in 50 mM sodium phosphate, pH 7.1, 40 degrees Celsius) {ECO:0000269|PubMed:15491996, ECO:0000269|PubMed:16301312}; KM=18.5 mM for X6 (in 50 mM sodium phosphate, pH 7.1, 40 degrees Celsius) {ECO:0000269|PubMed:15491996, ECO:0000269|PubMed:16301312}; KM=4.3 mM for X3 (in 0.1 M MOPS, pH 7.0, 30 degrees Celsius) {ECO:0000269|PubMed:15491996, ECO:0000269|PubMed:16301312}; pH dependence: Optimum pH is 6.2-7.3. Stable between pH 5.0 and 9.8 for 30 min at 30 degrees Celsius. {ECO:0000269|PubMed:15491996, ECO:0000269|PubMed:16301312}; Temperature dependence: Optimum temperature is 50 degrees Celsius. Stable up to 40 degrees Celsius. {ECO:0000269|PubMed:15491996, ECO:0000269|PubMed:16301312};

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family. {ECO:0000255}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125).
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.



Function:		Hydrolyzes xylooligosaccharides with a degree of polymerization of greater than or equal to 3, releasing xylose from the reducing end. Only hydrolyzes the beta anomers of xylooligosaccharides, with inversion of anomeric configuration. Hydrolyzes the glucose and xylose-based trisaccharides where xylose is located at the -1 subsite, GXX, XXG and GXG. Does not hydrolyze xylan, chitosan, lichenan, curdlan or carboxymethylcellulose. {ECO:0000269\|PubMed:15491996}.


Catalytic activity:		Reaction=Hydrolysis of (1->4)-beta-D-xylose residues from the reducing end of oligosaccharides.; EC=3.2.1.156; Evidence={ECO:0000269\|PubMed:15491996};
Biophysicochemical properties:		Kinetic parameters: KM=2.4 mM for X3 (in 50 mM sodium phosphate, pH 7.1, 40 degrees Celsius) {ECO:0000269\|PubMed:15491996, ECO:0000269\|PubMed:16301312}; KM=5.0 mM for X4 (in 50 mM sodium phosphate, pH 7.1, 40 degrees Celsius) {ECO:0000269\|PubMed:15491996, ECO:0000269\|PubMed:16301312}; KM=4.4 mM for X5 (in 50 mM sodium phosphate, pH 7.1, 40 degrees Celsius) {ECO:0000269\|PubMed:15491996, ECO:0000269\|PubMed:16301312}; KM=18.5 mM for X6 (in 50 mM sodium phosphate, pH 7.1, 40 degrees Celsius) {ECO:0000269\|PubMed:15491996, ECO:0000269\|PubMed:16301312}; KM=4.3 mM for X3 (in 0.1 M MOPS, pH 7.0, 30 degrees Celsius) {ECO:0000269\|PubMed:15491996, ECO:0000269\|PubMed:16301312}; pH dependence: Optimum pH is 6.2-7.3. Stable between pH 5.0 and 9.8 for 30 min at 30 degrees Celsius. {ECO:0000269\|PubMed:15491996, ECO:0000269\|PubMed:16301312}; Temperature dependence: Optimum temperature is 50 degrees Celsius. Stable up to 40 degrees Celsius. {ECO:0000269\|PubMed:15491996, ECO:0000269\|PubMed:16301312};
Similarity:		Belongs to the glycosyl hydrolase 8 (cellulase D) family. {ECO:0000255}.