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UniProt functional annotation for P61875 | ||
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| UniProt code: P61875. |
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| Organism: | |
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus. |
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| Function: | |
In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity. {ECO:0000269|PubMed:12296822}. |
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| Catalytic activity: | |
Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7; |
| Activity regulation: | |
An 11-mer corresponding to the PIP-box of RfcL inhibits DNA synthesis. {ECO:0000269|PubMed:12296822}. |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:18355915}. |
| Biotechnology: | |
Because Pfu DNA polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, it is routinely used for PCR. It is sold by a number of companies including Promega, Stratagene and ThermoScientific. Various mutations to improve its abilities have been incorporated. {ECO:0000269|PubMed:23940661}. |
| Similarity: | |
Belongs to the DNA polymerase type-B family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.