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PDBsum entry 3a1e
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References listed in PDB file
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Key reference
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Title
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Nucleotide recognition by copa, A cu+-Transporting p-Type atpase.
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Authors
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T.Tsuda,
C.Toyoshima.
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Ref.
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Embo J, 2009,
28,
1782-1791.
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PubMed id
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Abstract
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Heavy metal pumps constitute a large subgroup in P-type ion-transporting
ATPases. One of the outstanding features is that the nucleotide binding N-domain
lacks residues critical for ATP binding in other well-studied P-type ATPases.
Instead, they possess an HP-motif and a Gly-rich sequence in the N-domain, and
their mutations impair ATP binding. Here, we describe 1.85 A resolution crystal
structures of the P- and N-domains of CopA, an archaeal Cu(+)-transporting
ATPase, with bound nucleotides. These crystal structures show that CopA
recognises the adenine ring completely differently from other P-type ATPases.
The crystal structure of the His462Gln mutant, in the HP-motif, a
disease-causing mutation in human Cu(+)-ATPases, shows that the Gln side chain
mimics the imidazole ring, but only partially, explaining the reduction in
ATPase activity. These crystal structures lead us to propose a role of the His
and a mechanism for removing Mg(2+) from ATP before phosphoryl transfer.
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