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PDBsum entry 349d
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References listed in PDB file
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Key reference
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Title
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X-Ray crystal structures of half the human papilloma virus e2 binding site: d(gaccgcggtc).
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Authors
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J.B.Finley,
M.Luo.
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Ref.
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Nucleic Acids Res, 1998,
26,
5719-5727.
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PubMed id
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Abstract
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The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing half
the human papilloma virus E2 binding site, has been solved from two crystals
grown at different ionic conditions (50 mM MgCl2and 50 mM spermine or 1.56 mM
MgCl2and 1.56 mM spermine). Despite the variation in salt concentration, the two
DNA structures are in a very similar, A-type DNA conformation, with helical axes
curving towards the major groove. Although the salt concentrations do not effect
the helical parameters or hydration to a large degree, there is a change in the
overall helical curvature; 18 degrees and 31 degrees for the low and high salt
structures, respectively. This curvature appears to be sequence specific and
biologically relevant when compared with similar DNA structures, including the
E2 binding site of a protein-DNA complex.
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Headers
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