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PDBsum entry 2zyt
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References listed in PDB file
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Key reference
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Title
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Snapshot of a michaelis complex in a sulfuryl transfer reaction: crystal structure of a mouse sulfotransferase, Msult1d1, Complexed with donor substrate and accepter substrate.
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Authors
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T.Teramoto,
Y.Sakakibara,
M.C.Liu,
M.Suiko,
M.Kimura,
Y.Kakuta.
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Ref.
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Biochem Biophys Res Commun, 2009,
383,
83-87.
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PubMed id
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Abstract
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We report the crystal structure of mouse sulfotransferase, mSULT1D1, complexed
with donor substrate 3'-phosphoadenosine 5'-phosphosulfate and accepter
substrate p-nitrophenol. The structure is the first report of the native
Michaelis complex of sulfotransferase. In the structure, three proposed
catalytic residues (Lys48, Lys106, and His108) were in proper positions for
engaging in the sulfuryl transfer reaction. The data strongly support that the
sulfuryl transfer reaction proceeds through an S(N)2-like in-line displacement
mechanism.
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