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UniProt functional annotation for Q5SJ47 | ||
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| UniProt code: Q5SJ47. |
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| Organism: | |
Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8). |
| Taxonomy: | |
Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus. |
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| Function: | |
Single-stranded-DNA-specific exonuclease acting in a 5' to 3' direction; has no detectable activity on ssRNA. {ECO:0000269|PubMed:21087930}. |
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| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:21087930}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:21087930}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:21087930}; Note=Requires a divalent cation; Mg(2+) > Mn(2+) > Co(2+). Structures with 1 Mg(2+), or 1 or 2 Mn(2+) were determined; the authors conclude 2 metal ions are required for activity. {ECO:0000269|PubMed:21087930}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.17 uM for ssDNA 6-mers {ECO:0000269|PubMed:21087930}; KM=0.084 uM for ssDNA 11-mers {ECO:0000269|PubMed:21087930}; KM=0.037 uM for ssDNA 21-mers {ECO:0000269|PubMed:21087930}; Note=Kcat for ssDNA remains unchanged as chain length increases from 6-mers to 21-mers.; |
| Subunit: | |
Monomer. Intact protein binds ss- but not dsDNA. {ECO:0000269|PubMed:20129927, ECO:0000269|PubMed:21087930}. |
| Similarity: | |
Belongs to the RecJ family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.