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UniProt functional annotation for Q9AEM1 | ||
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| UniProt code: Q9AEM1. |
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| Organism: | |
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae; Corynebacterium. |
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| Function: | |
Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. {ECO:0000269|PubMed:11565516}. |
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| Catalytic activity: | |
Reaction=GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate; Xref=Rhea:RHEA:10388, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526, ChEBI:CHEBI:37565, ChEBI:CHEBI:58189, ChEBI:CHEBI:58702; EC=4.1.1.32; Evidence={ECO:0000255|HAMAP-Rule:MF_00452}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_00452}; Note=Binds 1 Mn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00452}; |
| Pathway: | |
Carbohydrate biosynthesis; gluconeogenesis. {ECO:0000255|HAMAP-Rule:MF_00452}. |
| Subunit: | |
Monomer. {ECO:0000255|HAMAP-Rule:MF_00452, ECO:0000269|PubMed:18234538}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00452}. |
| Disruption phenotype: | |
Cells lacking this gene lead to the absence of PEP carboxykinase activity and the inability to grow on acetate or lactate. {ECO:0000269|PubMed:11565516}. |
| Miscellaneous: | |
The presence and the level of PEP carboxykinase activity has a strong influence on the biosynthesis of glutamate and a weak influence on the biosynthesis of lysine. {ECO:0000269|PubMed:11565516}. |
| Similarity: | |
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. {ECO:0000255|HAMAP-Rule:MF_00452}. |
Annotations taken from UniProtKB at the EBI.